是真的吗/Cas9 腺病毒

高效极速基因敲除神器-是真的吗/Cas9 腺病毒



      是真的吗/Cas9 简介

是真的吗 (clustered, regularly interspaced, short palindromic repeats)是一种来自细菌降解入侵的病毒 DNA 或其他外源 DNA 的免疫机制。目前,来自Streptococcus pyogenes 的是真的吗-Cas9系统应用最为广泛。Cas9 蛋白(含有两个核酸酶结构域,可以分别切割DNA 两条单链。Cas9首先与crRNA及tracrRNA结合成复合物,然后通过PAM序列结合并侵入DNA,形成RNA-DNA复合结构,进而对目的DNA双链进行切割,使DNA双链断裂。


通过基因工程手段对crRNA和tracrRNA进行改造,将其连接在一起得到sgRNA(single guide RNA)。融合的RNA具有与野生型RNA类似的活力,但因为结构得到了简化更方便研究者使用。通过将表达sgRNA的原件与表达Cas9的原件相连接,得到可以同时表达两者的质粒,将其转染细胞,便能够对目的基因进行操作。














   1、Laterodorsal tegmentum interneuron subtypes oppositely regulate olfactory cue-induced innate fear
     (2016年发表于Nature Neuroscience,作者:段树民)
   2Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury Involvement of PARK2-dependent mitophagy
     (2016年发表于Autophagy作者:Xiangnan Zhang
   3、SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain
     (2016年发表于Nature Communications,作者:陈义汉)
   4、TRB3 links insulin IGF to tumour promotion by interacting with p62 and impeding autophagic proteasomal degradations
     (2016年发表于Nature Communications,作者:胡卓伟)
   5Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK
     (2016年发表于Nature Communications,作者:刘明耀)
   6POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation
     (2015年发表于Nature Communications,作者:Yongzhong Liu)
   7LRP6 acts as a scaffold protein in cardiac gap junction assembly
     (2016年发表于Nature Communications,作者:陈义汉)
   8Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression
     (2016年发表于plos genetics,作者:Jun Yan )
   9Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy
     (2016年发表于Kidney International,作者:易凡
   10Roles of miR-1-1 and miR-181c in ventricular septal defects
     (2016年发表于International Journal of Cardiology,作者:Li J)
   11CCK-8S increased the filopodia and spines density in cultured hippocampal neurons of APP/PS1 and wild-type mice
     (2013年发表于Neuroscience Letters,作者:王钦文)
   12Interleukin-1β induces autophagy by affecting calcium homeostasis and trypsinogen activation in pancreatic acinar cells
     (2013年发表于Int J Clin Exp Pathol ,作者:赵青川)
   13MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31
     (2014年发表于Oncology Reports,作者:Chaoyin Jiang)
   14MicroRNA-216b/Beclin 1 axis regulates autophagy and apoptosis in human Tenon's capsule fibroblasts upon hydroxycamptothecin exposure
     (2014年发表于Experimental Eye Research,作者: Yingbin Ge)
   15Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy
     (2014年发表于Acta histochemica,作者: Qiuyue Wang)
   16construction of recombinant adenovirus vector containing hBMP2 and hVEGF165 genes and its expression in rabbit Bone marrow mesenchymal stem cells
     (2014年发表于Tissue and Cell,作者:Chun-yang Meng)
   17Ulinastatin Reduces the Resistance of Liver Cancer Cells to Epirubicin by Inhibiting Autophagy
     (2014年发表于plos one,作者:金钢、胡先贵)
   18MicroRNA-204-5pMediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas
   19Adenovirus siMDM2 and NDRG2 Gene Therapy Inhibits Cell Proliferation and Induces Apoptosis of Squamous Cell Carcinoma
     (2015年发表于Cell Biochemistry and Biophysics,作者:Li Zhang)
   20The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species
     (2015年发表于Oxidative Medicine and Cellular Longevity,作者:Xin Shen)
   21Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro
     (2015年发表于International Journal of Molecular Medicine,作者:Yong Wang)
   22SIRT6 suppresses phenylephrine-induced cardiomyocyte hypertrophy though inhibiting
     (2015年发表于Journal of Pharmacological Sciences,作者:刘培庆)
   23Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro
     (2015年发表于General and Comparative Endocrinology,作者:刘国文)
   24SIRT6 suppresses isoproterenol-induced cardiac hypertrophy through activation of autophagy
     (2015年发表于Translational Research,作者:刘培庆)
   25Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy
     (2016年发表于International Journal of Molecular Medicine,作者:Hong Wang)
   26Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats
     (2016年发表于Spinal Cord)
   27miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2
     (2016年发表于Cell Death and Disease,作者: Q Zhao)
   28Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes
     (2016年发表于Osteoarthritis and Cartilage,作者: Jian Zhang)
   29β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy
     (2016年发表于 Cell Death and Disease,作者:F Yi )
   30MicroRNA-30a downregulation contributes to chemoresistance of osteosarcoma cells through activating Beclin-1-mediated autophagy
     (2016年发表于Oncology Reports,作者:Lifeng Lao)
   31Luteolin alleviates postinfarction cardiac dysfunction by upregulating autophagy through Mst1 inhibition
     (2016年发表于J. Cell. Mol. Med.,作者:Dongdong Sun )
   32Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p
     (2016年发表于Biochemical and Biophysical Research Communications,作者: Jiahong Dong)
   33Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway
     (2015年发表于Tumor Biology,作者:Xinchen Sun)
   34Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p
     (发表于Biochemical and Biophysical Research Communications,作者:Jiahong Dong)
   35miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1
     (2016年发表于Biochemical and Biophysical Research Communications,作者:Jun Peng)
   36House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway
     (2016年发表于Biochem Biophys Res Commun,作者:Zhou H)
   37、构建大鼠白细胞介素1β 基因shRNA 腺病毒载体;
     (2015年发表于中国组织工程研究 )
   38Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1
     (2016年发表于Oncotarget,作者:Heng Ma)
   39A novel role of microRNA 17-5p in the modulation of circadian rhythm
     (2016年发表于Sci Rep,作者:Ji-Min Cao )
   40Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease
     (2016年发表于J Autoimmun,作者:Xiao Y)
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